太行山区酸枣叶化学成分的UPLC-QTOF-MS分析

为评价冀南太行山产酸枣叶的质量,本研究采用超高效液相色谱-串联四级杆-飞行时间质谱(UPLC-Q-TOF-MS)技术对其化学成分进行系统分析。选用反相WatersBEHAmide(100mm×2.1mm,1.7μm)色谱柱,乙腈与水(含0.5%甲酸)作为流动相二元线性梯度洗脱,检测波长340nm,流速0.20mL/min,柱温40℃;以G2Q-TOF联用系统进行检测。通过得到的样品电喷雾-质谱(ESI-MS)正离子和负离子扫描模式下的准分子离子峰、碎片信息、保留时间,并结合相关文献,从酸枣叶中共鉴定出53个化合物,其中包括黄酮10个、三萜皂苷14个、三萜酸13个、生物碱3个、脂肪酸和有机酸13个。分析结果表明,UPLC-Q-TOF-MS技术可快速、灵敏地鉴定冀南太行山区酸枣叶中的化学成分,可为有效控制该药材质量,为阐明其药效物质基础提供理论依据。

Analysis of chemical constituents in Ziziphus jujuba var. spinosa folium by UPLC-QTOF-MS

In order to evaluate the quality in Ziziphus jujuba var. spinosa folium from Taihang Mountain of Southern Hebei Province,the chemical compositions were analyzed by ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS).Waters BEH Amide(100 mm×2.1 mm,1.7 μm)chromatographic column and the G2 Q-TOF spectrometer were used to analyze the extract sample.The mobile phases used were acetonitrile and 0.5% formic acid in water.The analysis was conducted at a flow rate was 0.20 mL/min and detection wavelength of 340 nm.The column temperature was kept at 40 ℃.The ESI-MS positive and negative molecular ion peaks,product ions,the retention time and together with the references were used to tentatively identify the chemical constituents.The results indicated that 53 metabolites from ethanol extract of Ziziphus jujuba var. spinosa folium were identified,including 10 flavones,14 terpenoid saponins,13 triterpenoids,3 alkaloids,13 fatty acids and organic acids.This method is rapid,sensitive,which can provide the reference for evaluation of the quality of the Ziziphus jujuba var. spinosa folium and clarifying the material basis.