红豆树叶挥发油化学成分及其抗氧化和抑菌活性研究

为探究红豆树叶挥发油的化学组成及其生物活性,本研究首次采用水蒸气蒸馏法提取红豆树叶挥发油,并通过气相色谱-质谱联用技术(GC-MS)分析其化学成分,结合DPPH和ABTS法及抑菌圈法,评价其体外抗氧化和抑菌活性。结果表明,从红豆树叶挥发油中共检测出化合物36个,占挥发油总量的90.50%;挥发油主要成分为1,4-二十烷二烯(25.72%)、1,19-二十烷二烯(10.85%)、2,6-二叔丁基对甲酚(10.14%)、邻苯二甲酸正丁基异丁基酯(9.75%)、(Z,Z)-6,9-二十烷二烯(7.60%)、(E,E)-α-金合欢烯(7.51%)、叶醇(4.74%)和2-异丙烯基-5-甲基-6-庚烯-1-醇(4.04%)。红豆树叶挥发油对DPPH自由基和ABTS自由基清除作用的半数有效量(ED₅₀)分别为0.27、0.14 mg/mL,且抗氧化活性与挥发油浓度呈量效相关。红豆树叶挥发油浓度为7.1 mg/mL时,其对枯草芽孢杆菌(Bacillus subtilis)、金黄色葡萄球菌(Staphylococcus aureus)、绿脓杆菌(Pseudomonas aeruginosa)和大肠杆菌(Escherichia coli)的抑菌圈分别为11.29、9.88、10.85和11.03 mm。本研究为红豆树叶资源的综合开发利用提供了理论基础。

Chemical constituents of the volatile oil from Ormosia hosiei leaves and its antioxidant and antimicrobial activity

In this study,we firstly reported that the volatile oil from Ormosia hosiei Hemsl. Et Wils. leaves was extracted by water-steam distillation and then analyzed by gas chromatography-mass spectrometry ( GC-MS). The antioxidant activity of the volatile oil was determined by free radical scavenging assays against 1,1-diphenyl-2-picrylhydrazine ( DPPH) and 2,2'-azino- bis-( 3-ethylbenzothiazoline-6-sulphonic acid)( ABTS). And its antimicrobial activity was also examined by inhibition zone. The results showed that thirty-six compounds,which represented 90. 50% of the total constituents,were identified from the volatile oil. The major compounds of the volatile oil were 1,4-eicosadiene ( 25. 72%),1,19-eicosadiene ( 10. 85%),2,6-di- tert-butyl-4-methylphenol ( 10. 14%),butyl isobutyl phthalate ( 9. 75%),( Z,Z)-6,9-eicosadiene ( 7. 60%),( E,E)-α-far- nesene ( 7. 51%),leaf alcohol ( 4. 74%) and 2-isopropenyl-5-methyl-6-hepten-1-ol ( 4. 04%). The volatile oil exhibited sig- nificant antioxidant activities against DPPH and ABTS free radicals in a concentration-dependent fashion,with the median ef- fective dose ED50 values of the antioxidant activities against DPPH and ABTS free radicals were 0. 27 mg /mL and 0. 14 mg / mL,respectively. When the concentration of the volatile oil was 7. 1 mg /mL,the inhibition zone of Bacillus subtilis,Staphylo- coccus aureus,Escherichia coli,and Pseudomonas aeruginosa was 11. 29,9. 88,10. 85,and 11. 03 mm,respectively. These re- sults provide theoretical basis for the comprehensive development and utilization of the natural resources of O. hosiei Hemsl. Et Wils.