Sequence-based genetic markers for genes and gene families: single-strand conformational polymorphisms for the fatty acid synthesis genes of Cuphea |
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Authors: | M B Slabaugh G M Huestis J Leonard J L Holloway C Rosato V Hongtrakul N Martini R Toepfer M Voetz J Schell S J Knapp |
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Institution: | (1) Department of Crop and Soil Science, Oregon State University, Corvallis, Oregon, 97331 USA, US;(2) Max Planck Institut fuer Zuechtungsforschung, 30 Carl-von-Linne Weg, D-50829 Cologne, Germany, DE |
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Abstract: | Gene sequences are rapidly accumulating for many commercially and scientifically important plants. These resources create
the basis for developing sequence-based markers for mapping and tracking known (candidate) genes, thereby increasing the utility
of genetic maps. Members of most of the gene families underlying the synthesis of seed oil fatty acids have been cloned from
the medium-chain oilseed Cuphea. Allele-specific-PCR (AS-PCR) and single-strand conformational polymorphism (SSCP) markers were developed for 22 fatty acid
synthesis genes belonging to seven gene families of Cuphea using homologous and heterologous DNA sequences. Markers were developed for 4 fatty-acyl-acyl carrier protein thioesterase,
2 β-ketoacyl-acyl carrier protein synthase I, 4 β-ketoacyl-acyl carrier protein synthase II, 3 β-ketoacyl-acyl carrier protein
synthase III, 3 acyl carrier protein, 2 β-ketoacyl-acyl carrier protein reductase, and 4 enoyl-acyl carrier protein reductase
loci. Eighty-eight percent (14 of 16) of the SSCP loci were polymorphic, whereas only 9% (2 of 22) of the AS-PCR loci were
polymorphic. These markers were mapped using a Cuphea viscosissima×C. lanceolata F2 population and produced linkage groups of 10, 3, and 2 loci (3 loci segregated independently). The 10-locus linkage group
had every gene but one necessary for the synthesis of 2- to 16-carbon fatty acids from acetyl-CoA and malonyl-ACP (the missing
gene family was not mapped). SSCP analysis has broad utility for DNA fingerprinting and mapping genes and gene families.
Received: 3 May 1996 / Accepted: 30 August 1996 |
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Keywords: | Cuphea β -Ketoacyl-ACP synthase Acyl-ACP thioesterase Medium-chain fatty acids |
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