Effects of hypobaric growth conditions on morphogenic potential and antioxidative enzyme activities in Saussurea involucrata |
| |
Authors: | B Guo B H Abbasi and Y H Wei |
| |
Institution: | (1) Phytochemical Engineering Group, National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100080, P.R. China;(2) Graduate School of the Chinese Academy of Sciences, Beijing, 100049, P.R. China; |
| |
Abstract: | Effects of reduced atmospheric pressure on morphogenic potential and antioxidative enzyme activities in regenerated tissues
of Saussurea involucrata were evaluated. Leaf explants were cultured at atmospheric pressure 30, 60 or 101 kPa on Murashige and Skoog (MS) medium
with several plant growth regulators (PGRs). Oxygen and carbon dioxide partial pressures were maintained at 21 and 0.038 kPa,
respectively. At 60 kPa, 12 shoots per explant were recorded, which was 1.5 and 2.1-folds higher than at 101 and 30 kPa, respectively.
A shooting frequency of 80 % was observed at 60 and 101 kPa. Rooted plantlets were obtained on MS medium with indoleacetic
acid. At 30, 60 and 101 kPa, rooting of shoots was 49, 72 and 85.6 %, respectively. The rooted plantlets were successfully
acclimatized to soil. Activities of all of antioxidative enzymes determined in present study were affected by hypobaric conditions. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|