Adaptive and Cross-Protective Responses of Pseudomonas sp. DJ-12 to Several Aromatics and Other Stress Shocks |
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Authors: | Sang-Ho Park Kye-Heon Oh Chi-Kyung Kim |
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Institution: | (1) Department of Microbiology and Biotechnology, and Research Institute for Genetic Engineering, Chungbuk National University, Cheongju 361-763, Korea, KR;(2) Department of Life Science, Soonchunhyang University, Asan, Chung-Nam, 336-600, Korea, KR |
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Abstract: | Pseudomonas sp. DJ-12 cells were subjected to mild treatments of stress such as exposure to biphenyl, 4-chlorobiphenyl (4CB), 4-hydroxybenzoate
(4HBA), ethanol, and heat, and then were examined for production of stress-shock proteins and morphological changes. The adapted
cells were then subjected to lethal stress conditions such as 200 mm 4CB, 100 mm biphenyl, 10 mm 4HBA, 20% ethanol, and 46°C
to examine crossly protective responses to the stresses. Several stress-shock proteins including DnaK and GroEL were newly
synthesized in the adapted cells. Some of them were commonly produced by those stresses separately treated. The cells treated
with these aromatic hydrocarbons showed destructive openings on the cell envelopes. On the other hand, those cells treated
with ethanol or heat displayed irregular rod shapes with wrinkled surfaces. The adapted cells to each stress under sublethal
conditions exhibited increased resistance to the same stress of lethal conditions. The cells adapted with 5 mm 4HBA showed
greater protection for survival than those adapted by other stresses. In addition, those adapted cells showed increased resistance
to other stresses as a cross-protection phenomenon. The cells adapted to 42°C exhibited markedly increased resistance to the
lethal stresses of 46°C as well as to 20% ethanol.
Received: 20 December 2000/Accepted: 26 January 2001 |
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