Optimum Conditions for Transformation of Maleic Acid by Immobilized Cells of Alcaligenes xylosoxidanssubsp. xylosoxidans260 |
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Authors: | I Yu Safronova E V Semenova |
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Institution: | (1) Biological Department, Lomonosov Moscow State University, Moscow, Russia |
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Abstract: | Immobilized cells of Alcaligenes xylosoxidanssubsp. xylosoxidans260 transformed 98% of the maleic acid (initial concentration of 5.0 g/l medium) under periodic conditions for 48 h. Free cells transformed only 26% of the substrate in 96 h. Immobilized cells of a selected S-variant ofA. xylosoxidanstransformed the maleate (30.0 g/l) entirely in 96 h during batch cultivation and only 15.0 g/l of the maleate in continuous cultivation at a flow rate of 0.03 h–1. |
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