Aurora-A高表达对食管癌细胞侵袭和 MMP-9表达的影响

目的:建立Aurora-A高表达的稳定细胞系,并探讨Aurora-A高表达对食管癌细胞侵袭和MMP-9蛋白表达与活性的影响。方法:利用脂质体法稳定转染Aurora-A表达质粒,通过Westernblot和RT-PCR鉴定Aurora-A高表达的稳定细胞系。利用细胞体外侵袭实验观察细胞的侵袭能力,并通过Western blot、ELISA和明胶酶谱的方法观察MMP-9的蛋白表达水平和活性。结果:建立了Aurora-A高表达的稳定细胞系,且Aurora-A高表达能增加食管癌细胞的体外侵袭能力,并提高了MMP-9的蛋白表达水平和活性。结论:初步阐明了Aurora-A促进食管癌细胞侵袭的作用以及分子机制,为Aurora-A可能成为治疗食管癌的有效靶点提供了依据。

Effect of Aurora-A Overexpression on Invasion and MMP-9 Expression of Esophageal Carcinoma Cells

Objective:To establish Aurora-A overexpressing transfectant clones and investigate its effect on invasiveness as well as protein expression and activity of MMP-9 in esophageal carcinoma cells.Methods:The Aurora-A expressing plasmid was transfected using LipofectamineTM 2000 and the identification of transfected clones was confirmed by Western blot analysis and RT-PCR.The invasive properties of cells were examined by an in vitro Boyden chamber invasion assay.Level of MMP-9 protein expression and activity were measured with Western blot and ELISA as well as gelatin zymography analysis.Results:Aurora-A overexpressing transfectant clone was established,which increased cell invasion as well as protein expression and activity of MMP-9.Conclusion:The elucidation the molecular mechanism for the promotion of invasion in esophageal carcinoma by Aurora-A may lead to the development of new target for effective therapy.