大鼠骨髓源树突状细胞吞噬功能的超微结构观察

目的:利用透射电镜对体外扩增并纯化的大鼠骨髓来源树突状细胞的吞噬过程进行超微结构观察。方法:利用粘附法诱导分离大鼠骨髓细胞,延长树突状细胞的培养时间,在透射电镜下观察树突状细胞对坏死细胞碎片的吞噬过程。结果:树突状细胞识别坏死的细胞碎片后,伸出粗大突起将坏死的细胞碎片环形包裹吞噬;吞噬坏死细胞后,树突状细胞呈不规则形,细胞表面可见明显突起,消化过程中细胞内形成大量大小不等、电子密度不均一的次级溶酶体及髓鞘样膜结构。结论:树突状细胞对凋亡或坏死细胞的提呈对于保持机体内环境的稳定非常重要;利用树突状细胞的吞噬作用进行肿瘤免疫、自身免疫研究,将为树突状细胞临床治疗提供新的途径。

Ultrastructure Oberservation of Rat Bone Marrow Derived Dendritic Cells During Phagocytosis In Vitro

Objective: To observe the ultrastructural change of dendritic cells(DC) derived from rat bone marrow during phagocytosis in vitro.Methods: Immunocyto-adherence test was used to get DC from rat bone marrow.By prolonging the culture time,the ultrastructural change of DC during phagocytosis was observed using transmis sion electron microcopy.Results: After recognizing the apoptotic cells,DC engulfed the cells with dendrites and became irregularly shaped.During the digestive process,a large amount of secondary lysosomes with heterogeneous electron density and myelin-like membrane structures with different sizes were observed in the DC.Conclusion: The uptake of apoptotic/necrotic cells by DC is crucial for maintaing the homeostasis.This experiment laid a foun dation for the further study of DC’s phagocytosis in tumor immunity and autoimmune.