响应面法优化木薯淀粉发酵生产单细胞油脂工艺

采用响应面分析法对发酵性丝孢酵母菌株以木薯淀粉水解液为原料合成微生物油脂的培养条件进行优化。首先利用Plackett-Burman试验设计确定影响油脂产量的主要因素,在此基础上再利用Box-Behnken试验设计及响应面分析法对其进行条件优化。结果表明,发酵温度、C/N、pH对油脂产量具有显著影响,产油脂的最佳发酵条件为:发酵温度28.78°C、C/N 126.18、pH 6.69,油脂产量达到14.88g/L,比优化前提高了28.6%。同时,气相色谱分析表明,微生物油脂脂肪酸组成成分主要包括棕榈酸、硬脂酸、油酸、亚油酸酯等,是优良的生物柴油制备原料。

Optimization of Single Cell Oil Produced from Cassava Starch by Response Surface Methodology

Response surface methodology was applied to optimize the Trichosporon fermentans fermentation conditions for microbial lipids produced from hydrolysate of cassava starch.The Plackeet-Burman design was adopted to sort the important factors influencing the lipids yield,and the conditions of lipids production was further optimized by using Box-Behnken design and response surface methodology.The results showed that the temperature,C/N and pH could influence on the lipid yield significantly.The optimized conditions for lipid production were temperature of 28.79°C,C/N of 126.18 and pH of 6.69,and the lipids yield of 14.88g/L which was 28.6% higher than control.Moreover,gas chromatography analysis revealed that the microbial lipid from Trichosporon fermentans mainly included palmitic acid,stearic acid,oleic acid and linoleic acid and it was suggested to be used as an excellent feedstock for biodiesel production.