取样方式对大鼠皮瓣缺血再灌注损伤模型细胞因子测定的影响

目标:利用大鼠腹岛状皮瓣构建缺血再灌注损伤模型,评价距血管夹闭点近、中、远不同距离的位置取样是否会影响细胞因子的检测,为该模型实验确立一种合理的取样方式。方法:对术后5天的大鼠成活皮瓣按照近、中、远三个位置进行取样,分别测定各样品的4种细胞因子IL-1β,IL-6,TNF-α和EPO的表达量,并用配对T检验分析评价三种取样方式。结果:样品中IL-1β,IL-6,TNF-α和EPO在缺血再灌注组的表达水平较假手术组显著上调;同一细胞因子在同一皮瓣不同取样位置的表达量在统计学上无显著性差异。结论:取样位置并不影响细胞因子的测定,即可以从成活皮瓣的任意位置取样测定相应细胞因子,但是在同一批实验中不同皮瓣取样位置须一致。

The Impact of Sampling on Cytokines Test of Rat Skin Flap Model

Aim: Construct rat skin flap model for the research of ischemia/reperfusion injury.The flap samples were harvested from the proximal,middle,and distal part of the viable flap and cytokines expression were detected separately in order to assess whether there were significant differences among the three parts and confirm an equitable way of sampling on ischemia/reperfusion injury of rat skin flap model.Method: Samples were harvested on postoperative day 5.The level of IL-1β,IL-6,TNF-α and EPO were detected to assess the sampling way.Statistical analyses were performed using SPSS.Results:Significant difference in cytokines levels was noted between the two groups.But no statistical difference in cytokines levels in either inter-group.Conclusion:Samples can be harvested at any arbitrary point on a skin flap to stand for the inflammatory level of the whole flap.And it should be sampled from the same position on different flaps in the same experiment.However harvest from the proximal point were encouraged for taking parallel samples.