| 重组腺相关病毒生产方法研究进展 |
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| 引用本文： | 武虹宇,吕颖慧,许瑞安,王启钊.重组腺相关病毒生产方法研究进展[J].生物技术通讯,2012,23(4):595-600. |
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| 作者姓名： | 武虹宇 吕颖慧 许瑞安 王启钊 |
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| 作者单位： | 华侨大学分子药物学研究所,分子药物教育部工程研究中心,福建泉州 362021 |
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| 基金项目： | 国家自然科学基金(30900822);福建省发改委项目(2009年第2批[26]);福建省高校杰出青年人才计划 |
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| 摘 要： | 重组腺相关病毒(rAAV)作为基因治疗的载体,具有感染范围广、能在宿主细胞中长久稳定表达外源基因和非致病性等优点,因此在基因治疗领域倍受青睐。rAAV的大规模制备技术一直是限制其临床广泛应用的瓶颈。近年来,有许多改进rAAV制备工艺的尝试。我们在介绍rAAV载体制备所需各种元件的基础上,对这些方法进行了简要综述。
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| 关 键 词： | 腺相关病毒 基因治疗 规模化制备 |
Improvement of Recombination Adeno-Associated Virus Production |
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| WU Hong-Yu
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L Ying-Hui
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XU Rui-An
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WANG Qi-Zhao.Improvement of Recombination Adeno-Associated Virus Production[J].Letters in Biotechnology,2012,23(4):595-600. |
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| Authors: | WU Hong-Yu L Ying-Hui XU Rui-An WANG Qi-Zhao |
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| Institution: | * Institute of Molecular Medicine,Huaqiao University;Molecular Medicine Engineering Research Center,Ministry of Education;Quanzhou 362021,China |
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| Abstract: | Recombinant adeno-associated virus(rAAV) vectors have shown great promise for gene therapy due to its broad tropism and safety.The major bottleneck to broadening the usage of rAAV vectors remains the limit ed capacity of available production systems to provide sufficient rAAV quantities for preclinical and clinical trials.Recently,some attempts of improvement for production of rAAV have been developed.We summarized the follow ing protocols based on the introduction of the elements which are required in rAAV vector manufacture. |
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| Keywords: | adeno-associated virus gene therapy large-scale production |
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