重组大肠杆菌菌蜕与幽门螺杆菌蛋白质疫苗协同免疫BALB/c小鼠

目的:考察重组菌蜕和蛋白质疫苗混合后对BAB/c小鼠机体免疫反应的影响。方法:首先构建展示尿素酶B抗原表位的大肠杆菌菌蜕,同时通过融合PCR构建分子内佐剂蛋白质疫苗rLTBKAT,然后分别利用该菌蜕和蛋白质疫苗免疫BAB/c小鼠。结果:当rLTBKAT和重组菌蜕联合口服免疫BAB/c小鼠时,其抗尿素酶B抗体的效价由单独免疫时的1∶(239±23)提高到1∶(681±76),二者差异极显著(P=0.009);而其抗过氧化氢酶抗体的效价则由单独免疫时的1∶(2800±275)下降至1∶(1800±400),二者差异不显著(P=0.08)。抗体分型试验表明,单独和联合免疫时,其抗尿素酶B抗体类型均以IgG1为主,而抗过氧化氢酶抗体类型则由单独免疫时的IgG1为主转变为联合免疫时的IgG2a为主。联合免疫时,小鼠抗尿素酶B和抗过氧化氢酶IgA抗体水平均高于单独免疫时。结论:重组菌蜕和蛋白质疫苗联合免疫,可提高机体对某些抗原的免疫水平或改变其反应类型。

Cooperative Vaccination of BLAB/c Mice with Bacterial Ghosts Dis playing UreB Epitope of Helicobacter pylor and Recombinant Pro tein LTBKAT

Objective: To study the immune response of BLAB/c mice to vaccination of recombinant bacterial ghosts cooperated with protein vaccine.Methods: The bacterial ghost displaying UreB epitope as well as recombi nant LTBKAT were constructed,and then they were mixed or separately orally administrated by BLAB/c mice.Re sults: The anti-UreB antibody titer of mice immunized with mixed vaccine was 1∶(681±76),which was significant ly higher than that immunized with single bacterial ghosts[1∶(239±23)].The anti-UreB antibody type of both groups was mainly IgG1.The anti-KatA antibody titer of mice immunized with mixed vaccine was 1∶(1800±400),which was lower than that immunized with single protein vaccine[1∶(2800±275)].The anti-KatA antibody type of mixed vaccination was IgG2a,which was very different from that of protein vaccination.The anti-UreB and an ti-KatA IgA antibody level of mixed vaccination were both higher than those of single immunization.Conclusion: Immunogenicity level of BLAB/c mice was improved,even immunogenicity type was changed.