机械拉伸对骨髓间充质干细胞迁移行为的影响

目的:研究机械拉伸刺激对大鼠骨髓间充质干细胞迁移行为的影响并探讨其相关分子机制。方法:应用单轴机械拉伸加载装置考察不同条件的周期拉伸刺激对大鼠骨髓间充质干细胞迁移行为的影响,采用Transwell和划痕法评价细胞迁移能力,采用明胶酶谱法检测基质金属蛋白酶-2,-9(MMP-2,-9)表达的变化。结果:适宜的拉伸刺激可以明显促进大鼠骨髓间充质干细胞的迁移能力,1 Hz、10%应变拉伸8 h后可以使细胞迁移数量增加到对照组的1.58倍。拉伸刺激诱导骨髓间充质干细胞基质金属蛋白酶-2,-9(MMP-2,-9)表达。抑制剂GM6001抑制了拉伸诱导的MMP-2,-9分泌增加,同时抑制了拉伸刺激对细胞迁移的促进作用。结论:机械拉伸刺激影响大鼠骨髓间充质干细胞的迁移行为,MMP-2,-9在此过程中可能起着重要介导作用。

Effect of Mechanical Stretch on Migration of Bone Mesenchymal Stem Cells in vitro

Objective: To investigate the effects of the cyclic stretch on rat bone marrow mesenchymal stem cells(BMSCs) migration and its molecular mechanism.Methods: The cyclic mechanical stretching apparatus were used to study the effects of the cyclic stretch on the migration of BMSCs with different parameters.Transwell and scratching method was used to detect the number of cell migration and the levels of matrix metallopoteinases-2,-9 proteins were detected by zymography.Results: The migration of BMSCs could be remarkably stimulated by appropriate cyclic stretch,and the number of cells was increased to 1.58 times compared with that of the control group at 1 Hz,10% strain for 8 hours.Moreover,the expression level of matrix metallopoteinases-2,-9 protein was increased significantly after the stimulation of the stretch.After treated with GM6001,the secretion of MMP-2,-9 and the migration of BMSCs stimulated by the cyclic stretch was inhibited prominently.Conclusions: Matrix metallopoteinases-2,-9 play an important role in the cours that the cyclic stretch regulated on the migration of BMSCs.