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| PCR鉴定肺炎克雷伯菌的强毒性血清型 | |
| 引用本文： | 和晋渝,邱景富,刘梦颖,闫小娟,李迎丽,郭兆彪,周冬生.PCR鉴定肺炎克雷伯菌的强毒性血清型[J].生物技术通讯,2012,23(4):554-557. |
| 作者姓名： | 和晋渝 邱景富 刘梦颖 闫小娟 李迎丽 郭兆彪 周冬生 |
| 作者单位： | 1. 重庆医科大学公共卫生与管理学院,重庆,400016 2. 重庆医科大学公共卫生与管理学院,重庆 400016;军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京 100071 3. 军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京 100071 |
| 基金项目： | 国家自然科学基金(31071093,31170129);国家重点基础研究发展计划(2009CB522600) |
| 摘 要： | 目的:了解肺炎克雷伯菌强毒性血清型K1、K2、K54和K57型菌株在我国重庆、北京、深圳三地的分布及流行趋势。方法:采用PCR对310株肺炎克雷伯菌临床分离株进行血清型K1、K2、K54和K57检测。结果:310株菌中,K1、K2、K54和K57血清型分别占14.2%、9.4%、6.5%和4.2%;来自呼吸系统标本分离株中的K1血清型菌株在4种检测的强毒血清型中占首位,为呼吸系统总数的17.4%。结论:310株肺炎克雷伯菌的4种强毒性血清型中,K1血清型菌株所占比例高,较为流行。 |
| 关 键 词： | 肺炎克雷伯菌 血清型 强毒株 聚合酶链反应 |
Polymerase Chain Reaction Analysis for Detecting Highly Virulent Serotypes of Klebsiella pneumoniae |
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| HE Jin-Yu , QIU Jing-Fu , LIU Meng-Ying , YAN Xiao-Juan , LI Ying-Li , GUO Zhao-Biao , ZHOU Dong-Sheng.Polymerase Chain Reaction Analysis for Detecting Highly Virulent Serotypes of Klebsiella pneumoniae[J].Letters in Biotechnology,2012,23(4):554-557. | |
| Authors: | HE Jin-Yu QIU Jing-Fu LIU Meng-Ying YAN Xiao-Juan LI Ying-Li GUO Zhao-Biao ZHOU Dong-Sheng |
| Institution: | 1.School of Public Health and Management,Chongqing Medical University,Chongqing 400016;2.State Key Labo ratory of Pathogenic Microbe and Biosecurity,Institute of Microbiology and Epidemiology,Academy of Military Med ical Sciences,Beijing 100071;China |
| Abstract: | Objective: To characterize the distribution of highly virulent serotypes of Klebsiella pneumoniae in Chongqing,Beijing and Shenzhen city in China.Methods: Genomic DNA was extracted,and then four serotypes related to the highly virulent strains were screened in a collection of 310 strains of K.pneumoniae by polymerase chain reaction(PCR).Results: Serotypes K1,K2,K57 and K54 accounted for 14.2%,9.4%,6.5% and 4.2% of the 310 isolates tested.Compared to the other three serotypes,the serotype K1 occurred most frequently in the strains from the respiratory system.Conclusion: K1 serotype is predominant in the 310 strains of K.pneumoniae in relative to the other serotypes tested. |
| Keywords: | Klebsiella pneumoniae serotyping highly virulent polymerase chain reaction |
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