哺乳动物精子磷酸化蛋白质组学研究进展

蛋白质的表达、修饰及相互作用的研究已成为后基因组学时代蛋白质组学中的重要内容。蛋白质磷酸化和去磷酸化作为最普遍的翻译后修饰之一，是精子细胞信号转导和酶调控、表达的主要分子机制，亦是精子、卵细胞信号识别及完成受精作用的关键环节。对精子磷酸化蛋白功能的研究有助于深入理解精子的获能、超激活运动的维持、发生顶体反应及精卵结合等受精过程的分子调控机理。对哺乳动物精子磷酸化蛋白质组学的研究进展，包括动物精子磷酸化蛋白质组学研究的技术方法、磷酸化蛋白质种类的鉴定、定量及其功能分析进行了综述，为进一步发掘与受精相关的重要生物标志物，揭示精子发育、繁殖潜能变化及受精分子机理奠定基础。

Resent Advances on Phosphoproteomics Researches of Mammalian Sperm Capacitation

Studies on protein expression,modification and interaction have become important in proteomics in the post-genomics era.Phosphorylation/dephosphorylation of proteins is the main mechanism of signal transduction and regulation of enzymes in sperm cells,what’s more,it plays a key role during the sperm-egg recognition and fertilization process.Researches about function of phosphorylated proteins contribute to the understanding of the molecular mechanism of sperm capacitation,hyperactive motility and acrosome reaction process.The progress in the researches of mammalian sperm phosphoproteomics was reviewed that include the phosphoproteomics technology,phosphorylated proteins identification and quantitation,function analysis benefits in finding new important fertilization related biological markers and revealing the sperm development,reproductive potential changes and molecular mechanism of fertilization.