3种花色苷对DF-1细胞的影响

目的:探讨来自心里美萝卜、紫甘薯和紫玉米的3种不同的花色苷对DF-1细胞单层生长的影响。方法:直观观察3种花色苷对DF-1细胞生长的影响,初步摸索其最适终浓度;用MTT法检测花色苷在提高细胞活性方面的作用。结果:不同浓度的3种花色苷均对细胞单层生长有不同的影响,心里美萝卜花色苷、紫甘薯花色苷和紫玉米花色苷对细胞生长的最适终浓度分别为100、75和50μg/mL;用MTT法获得相应数据并制成细胞活性图表。结论:不同品种来源及不同浓度的花色苷对DF-1细胞单层生长均有明显影响,为今后开展花色苷促细胞生长,提高细胞抗病毒活性研究提供了数据基础。

Effect of Three Sorts of Anthocyanin on DF-1 Cells

Objective: To investigate the impact of three sorts of anthocyanin including heart radish anthocyanin,purple potato anthocyanin and purple corn anthocyanin on DF-1 cell line.Methods: The impact of the three sorts of anthocyanin on DF-1 cells growth was explored and the final concentration was measured respectively by intuitive observation.The impact of the three sorts of anthocyanin in improving the role of cell activity was ex plored by MTT assay.Results: The different concentrations of anthocyanins were affected on DF-1 monolayer cells growth,and the optimal final concentration of heart radish anthocyanin,purple potato anthocyanin and purple corn anthocyanin was got for 100,75 and 50 μg/mL respectively.At the same time,we mapped the chart based on the data measured by MTT assay.Conclusion: Different varieties of anthocyanins as different concentrations were affected on DF-1 monolayer cells growth significantly.These will provide data support for the research of an thocyanins antivirus in the future.