Expression of a synthetic gene encoding the sweet-tasting protein thaumatin in the filamentous fungus Penicillium roquefortii |
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| Authors: | I Faus C Pati-o JL del R'o C del Moral HS Barroso J Bladé V Rubio |
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| Institution: | 1. Laboratory of Biotechnology, URQUIMA, S.A. (URIACH GROUP), Arnau de Vilanova, 22–42, 08105, Sant Fost de Campsentelles (Barcelona), Spain
2. Centro Nacional de Biotecnolog'a (CSIC-UAM), Campus Canto Blanco, 28049, Madrid, Spain
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| Abstract: | A synthetic gene encoding the amino acid sequence of the sweet-tasting protein thaumatin II has been expressed in Penicillium roquefortii. Using several different expression cassettes thaumatin was expressed either intracellularly or extracellularly, at concentrations of 1–2 mg thaumatin/l. |
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