Successful cryopreservation of suspension cells by encapsulation-dehydration |
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Authors: | Y Bachiri C Gazeau J Hansz C Morisset J Dereuddre |
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Institution: | (1) Cryobiologie Végétale, Université Pierre et Marie Curie, 12 Rue Cuvier, 75252 Paris Cedex 05, France |
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Abstract: | Cryopreservation of a Catharanthus cell suspension was performed after encapsulation in alginate beads. Encapsulated cells were precultured in sucrose-enriched medium for several days, dried over silica gel, and directly cooled in liquid nitrogen. After rewarming in air at room temperature, alginate beads were placed on semi-solid culture medium. Following regrowth, beads transferred to liquid medium generated a new cell suspension. Cell survival and regrowth from cryopreserved encapsulated cells depended on preculture duration and residual water content after air-drying.Jean Dereuddre unexpectedly passed away on 16 February 1995. |
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Keywords: | Alginate dehydration Catharanthus roseus cell suspension cryopreservation encapsulation |
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