| 红豆草抗羟脯氨酸变异系的筛选及其特性研究 |
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| 引用本文： | 赵相山,贾敬芬. 红豆草抗羟脯氨酸变异系的筛选及其特性研究[J]. 分子细胞生物学报, 1993, 0(3) |
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| 作者姓名： | 赵相山 贾敬芬 |
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| 作者单位： | 兰州大学细胞生物学研究室 730000(赵相山)，兰州大学细胞生物学研究室 730000(贾敬芬) |
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| 摘 要： | 通过选择-NaN_3诱变处理-再选择的程序从红豆草下胚轴愈伤组织获得了抗脯氨酸类似物L-羟脯氨酸的变异细胞系。抗性变异细胞系的游离脯氨酸含量比正常细胞系高4.6倍,并且抗性变异系亦表现出了很强的耐NaCl、PEG 能力。这可能为筛选抗逆境植物提供一条有效途径。
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| 关 键 词： | 红豆草 组织培养 变异系 L-羟脯氨酸 氯化钠抗性 聚乙二醇抗性 |
SELECTION AND CHARACTERIZATION OF CULTURED SAINFOIN(ONOBRYCHIS VICIAEFOLIA SCOP.)CALLUS RESISTANT TO HYDROXYPROLINE |
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| Zhao Xiang-shan Jia Jing-fen. SELECTION AND CHARACTERIZATION OF CULTURED SAINFOIN(ONOBRYCHIS VICIAEFOLIA SCOP.)CALLUS RESISTANT TO HYDROXYPROLINE[J]. Journal of Molecular Cell Biology, 1993, 0(3) |
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| Authors: | Zhao Xiang-shan Jia Jing-fen |
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| Abstract: | Hydroxyproline-resistant cell line wasselected from callus of Onobrychis viciaefo-lia scop by selection-NaN_3 treatment-rese-lection system.The variant line containedmore than 4.6 times the wild type levelof free proline when callus were grownaway from HYP for three months.The to-tal free amino acids content was also in-creased.When tested on media with inhi-bitory NaCl,PEG concentrations,the va-riant line showed better tolerance thanwild type line.This work indicates thatthe procedure used in our experiment mig-ht be an effective approach for selectingstress tolerant plant. |
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| Keywords: | Sainfoin (Onobrychis viciaefolia stop).Tissue culture.Variant line.Lhydroxyproline.NaCl toleranoe.PEG tolerance. |
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