Involvement of nitric oxide in cerebroside-induced defense responses and taxol production in <Emphasis Type="Italic">Taxus yunnanensis</Emphasis> suspension cells |
| |
Authors: | Jian Wen Wang Li Ping Zheng Ren Xiang Tan |
| |
Institution: | (1) School of Pharmaceutical Sciences, Soochow University, Suzhou, 215123, People’s Republic of China;(2) Department of Horticultural Sciences, Soochow University, Suzhou, 215123, People’s Republic of China;(3) Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210093, People’s Republic of China |
| |
Abstract: | This work was to characterize the generation of nitric oxide (NO) in Taxus yunnanensis cells induced by a fungal-derived cerebroside and the signal role of NO in the elicitation of plant defense responses and
taxol production. (2S,2′R,3R,3′E,4E,8E)-1-O-β-d-glucopyranosyl-2-N-(2′-hydroxy-3′-octadecenoyl)-3-hydroxy-9-methyl-4,8-sphingadienine at 10 μg/ml induced a rapid and dose-dependent NO production
in the Taxus cell culture, reaching a maximum within 5 h of the treatment. The NO donor sodium nitroprusside (SNP) potentiated cerebroside-induced
H2O2 production and cell death. Inhibition of nitric oxide synthase activity by phenylene-1,3-bis(ethane-2-isothiourea) dihydrobromide
or scavenging NO by 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide partially blocked the cerebroside-induced
H2O2 production and cell death. Moreover, NO enhanced cerebroside-induced activation of phenylalanine ammonium-lyase and accumulation
of taxol in cell cultures. These results are suggestive of a role for NO as a new signal component for activating the cerebroside-induced
defense responses and secondary metabolism activities of plant cells.
Taxol is a trademark of Bristol-Myers Squibb, Madison, NJ. |
| |
Keywords: | Cerebroside Nitric oxide Oxidative responses Phenylalanine ammonium-lyase Taxol production Taxus yunnanensis |
本文献已被 PubMed SpringerLink 等数据库收录! |
|