Stringent and reproducible tetracycline-regulated transgene expression by site-specific insertion at chromosomal loci with pre-characterised induction characteristics |
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| Authors: | Rachel Brough Antigoni M Papanastasiou Andrew CG Porter |
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| Affiliation: | (1) Gene Targeting Group, Department of Haematology and MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College, London, W12 0NN, UK;(2) The Breakthrough Breast Cancer Research Centre, Institute of Cancer Research, 237 Fulham Road, London, SW3 6JB, UK;(3) Institute of Child Health, 30 Guilford Street, London, WC1N 1EH, UK |
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| Abstract: | Background The ability to regulate transgene expression has many applications, mostly concerning the analysis of gene function. Desirable induction characteristics, such as low un-induced expression, high induced expression and limited cellular heterogeneity, can be seriously impaired by chromosomal position effects at the site of transgene integration. Many clones may therefore need to be screened before one with optimal induction characteristics is identified. Furthermore, such screens must be repeated for each new transgene investigated, and comparisons between clones with different transgenes is complicated by their different integration sites. |
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