Pioglitazone retrieves hepatic antioxidant DNA repair in a mice model of high fat diet |
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| Authors: | Pi-Jung Hsiao Tusty-Jiuan Hsieh Kung-Kai Kuo Wei-Wen Hung Kun-Bow Tsai Ching-Hsiu Yang Ming-Lung Yu Shyi-Jang Shin |
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| Institution: | (1) Division of Endocrinology and Metabolism, Department of Internal Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;(2) Graduate Institute of Medical Genetics, Kaohsiung Medical University, Kaohsiung, Taiwan;(3) Hepatobiliary Division, Department of Surgery, Kaohsiung Medical University, Kaohsiung, Taiwan;(4) Department of Pathology, Kaohsiung Medical University, Kaohsiung, Taiwan;(5) Hepatobiliary Division, Department of Internal Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan |
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| Abstract: | Background Pioglitazone was reported to improve hepatic steatosis and necroinflammation in human studies. To investigate whether the
hepato-protective effect of pioglitazone was associated with an improvement of antioxidant defense mechanism, oxidative DNA
damage and repair activity were determined in a high fat diet model. Male C57BL/6 mice were respectively fed with a 30% fat
diet, the same diet with pioglitazone 100 mg/kg/day, or a chow diet as control for 8 weeks. Tissue oxidative stress was indicated
by malondialdehyde concentration. Oxidative DNA damage was detected by immunohistochemical 8-oxoG staining. Enzymatic antioxidant
defense was detected by the real-time PCR of superoxide dismutase (Sod1, Sod2) and DNA glycosylase (Ogg1, MutY). Oxidative DNA repair was detected by immunohistochemical staining and western blotting of OGG1 expression. |
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