New probes used for IS1245 and IS1311 restriction fragment length polymorphism of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. hominissuis isolates of human and animal origin in Norway |
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| Authors: | Tone Bjordal Johansen Ingrid Olsen Merete Rus?s Jensen Ulf R Dahle Gudmund Holstad and Berit Dj?nne |
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| Institution: | (1) Department of Animal Health, National Veterinary Institute, PO Box 8156, 0033 Oslo, Norway;(2) Norwegian Institute of Public Health, PO Box 4404 Nydalen, 0403 Oslo, Norway;(3) Norwegian School of Veterinary Science, PO Box 8146, 0033 Oslo, Norway |
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| Abstract: | Background
Mycobacterium avium is an environmental mycobacterium that can be divided into the subspecies avium, hominissuis, paratuberculosis and silvaticum. Some M. avium subspecies are opportunistic pathogens for animals and humans. They are ubiquitous in nature and can be isolated from natural
sources of water, soil, plants and bedding material. Isolates of M. avium originating from humans (n = 37), pigs (n = 51) and wild birds (n = 10) in Norway were examined by IS1245 and IS1311 RFLP using new and specific probes and for the presence of IS901 and ISMpa1 by PCR. Analysis and generation of a dendrogram were performed with the software BioNumerics. |
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