A nanoplex PCR assay for the rapid detection of vancomycin and bifunctional aminoglycoside resistance genes in <Emphasis Type="Italic">Enterococcus</Emphasis> species |
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Authors: | Chan Yean Yean Lee Su Yin Pattabhiraman Lalitha Manickam Ravichandran |
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Institution: | (1) Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Malaysia;(2) School of Health Sciences, Universiti Sains Malaysia, Malaysia |
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Abstract: | Background Enterococci have emerged as a significant cause of nosocomial infections in many parts of the world over the last decade.
The most common enterococci strains present in clinical isolates are E. faecalis and E. faecium which have acquired resistant to either gentamicin or vancomycin. The conventional culture test takes 2–5 days to yield complete
information of the organism and its antibiotic sensitivity pattern. Hence our present study was focused on developing a nanoplex
PCR assay for the rapid detection of vancomycin and bifunctional aminoglycoside resistant enterococci (V-BiA-RE). This assay
simultaneously detects 8 genes namely 16S rRNA of Enterococcus genus, ddl of E. faecalis and E. faecium, aacA-aphD that encodes high level gentamicin resistance (HLGR), multilevel vancomycin resistant genotypes such as vanA, vanB, vanC and vanD and one internal control gene. |
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