Putative epidermal stem cell convert into corneal epithelium-like cell under corneal tissue <Emphasis Type="Italic">in vitro</Emphasis> |
| |
Authors: | Gao Nan Wang ZhiChong Huang Bing Ge Jian Lu Rong Zhang KeFei Fan ZhiGang Lu Li Peng Zhan Cui GuangHui |
| |
Institution: | 1. Key Laboratory of Ophthalmology Sun Yat-sen University, Ministry of Education 2. Key Laboratory of Ophthalmology (Sun Yat-sen University), Ministry of Education; Zhongshan Ophthalmic Center, Guangzhou 510060, China;Liwan Hospital of Guangzhou Medical College, Guangzhou 510165, China |
| |
Abstract: | Rhesus putative epidermal stem cells are being investigated for their potential use in regenerative corneal epithelium-like
cells, which may provide a practical source of autologous seed cells for the construction of bioengineered corneas. The goal
of this study was to investigate the potential of epidermal stem cells for trans-differentiation into corneal epithelium-like
cells. Rhesus putative epidermal stem cells were isolated by type IV collagen attachment method. Flow cytometry analysis,
immunohistology and RT-PCR were conducted to identify the expression of specific markers (β1m α6 integrin, K15, K1/K10, K3/K12
and CD71) on the isolated rapid attaching cells. The isolated cells were cocultured with human corneal limbal stroma and corneal
epithelial cells. After coculture, the expression of the same specific markers was evaluated in order to identify expression
difference caused by the coculture conditions. K3/K12 expression was analyzed in coculture cells on day 2, 4, 6, 8 and 10.
Putative epidermal stem cells in conditioned culture media were used as control. Putative epidermal stem cells were predominant
in rapid attaching cells by type IV collagen attachment isolation. Before being cocultured, the rhesus putative epidermal
stem cells expressed K15, α6 and β1 integrin, but no CD71, K1/K10 and K3/K12. After coculture, these cells expressed K3/K12
(a marker of corneal epithelial cells), K15 and β1 integrin, but no K1/K10. Cells being not coculture converted into terminally
differentiated cells expressing K1/K10. These results indicate that rhesus putative epidermal stem cells can trans-differentiate
into corneal epithelium-like cells and, therefore, may have potential therapeutic application as autologous seed cells for
the construction of bioengineered corneas. |
| |
Keywords: | epidermal stem cells corneal epithelium cells trans-differentiation |
本文献已被 万方数据 SpringerLink 等数据库收录! |
| 点击此处可从《中国科学C辑(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学C辑(英文版)》下载免费的PDF全文 |
|