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A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm |
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| Authors: | Pascal Philibert Audrey Stoessel Wei Wang Annie-Paule Sibler Nicole Bec Christian Larroque Jeffery G Saven Jérôme Courtête Etienne Weiss Pierre Martineau |
| Affiliation: | 1.CNRS,UMR5160, CRLC,Montpellier Cedex 5,France;2.CNRS,Illkirch,France;3.Department of Chemistry,University of Pennsylvania,Philadelphia,USA;4.Université de Montpellier I,Montpellier,France |
| Abstract: | BackgroundIntrabodies are defined as antibody molecules which are ectopically expressed inside the cell. Such intrabodies can be used to visualize or inhibit the targeted antigen in living cells. However, most antibody fragments cannot be used as intrabodies because they do not fold under the reducing conditions of the cell cytosol and nucleus. |
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