Degradation of triglycerides by a pseudomonad isolated from milk: molecular analysis of a lipase-encoding gene and its expression in Escherichia coli. |
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Authors: | L A Johnson I R Beacham I C MacRae M L Free |
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Affiliation: | School of Science, Griffith University, Nathan, Brisbane, Queensland, Australia. |
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Abstract: | Psychrotrophic lipolytic bacteria represent a significant problem in the storage of refrigerated dairy products. A lipase-encoding gene has been cloned and characterized from a highly lipolytic strain of Pseudomonas. The nucleotide sequence of the gene predicts a polypeptide of M(r) 49,905, which was identified when the gene was expressed in Escherichia coli. |
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