不同采血途径对SSD大鼠全血细胞计数的影响

为探讨不同采血途径对SD大鼠全血细胞计数的影响,采用全自动血液分析对46例正常SD大鼠的全血细胞计数(CBC)进行分析包括WBC、RBC、Hb、HCT、MCV、MCH、MCHC、PLT等指标;分别从尾静脉、眼眶静脉、颈动脉和腹主动脉取血,并以腹主动脉测值为参照进行配对t检验。结果显示:分别比较腹主动脉采血组与尾静脉采血组和眼眶静脉采血组,CBC值除MCV外其他值差异均有统计学意义(P<0.05);腹主动脉采血组和颈动脉采血组两者仅WBC差异有统计学意义(P<0.05)。以上结果说明不同采血途径对SD大鼠全血细胞计数有不同程度的影响,各实验室应建立各自的背景数据,以便能在统一条件下比对。

Influence of Different Sampling Ways on Blood Cell Parameters in Rat

The objective of the study is to discuss the differences of blood cell parameters in rat by different sampling ways. Blood cells collected from abdominal aorta, orbitalis vein, jugular aorta and tail vein of 46 healthy rats were detected by ABBOTT CD-3700sl Automatic Hematology Analyzer. The parameters of WBC, RBC, Hb, HCT, MCV, MCH, MCHC and PLT from different sampling ways were analyzed and compared with those from abdominal aorta using paired t test. The results were as follows: all the parameters in tail vein group and orbital vein group were significantly higher than those in abdominal aorta group ( P <0.01), except MCV. While only the WBC in jugular aorta group was different from that in abdominal aorta group ( P <0.05). As to complete blood cell, orbital vein and tail vein were significantly different from abdominal aorta, but the difference was minor between jugular aorta and abdominal aorta. In a word, blood sampling ways would have effect on CBC. So each laboratory should set up own data base to construct consistent background.