Proteomic analysis of Arabidopsis protein S-nitrosylation in response to inoculation with <Emphasis Type="Italic">Pseudomonas syringae</Emphasis> |
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| Authors: | Ana M Maldonado-Alconada Sira Echevarría-Zomeño Christian Lindermayr Inmaculada Redondo-López Jörg Durner Jesús V Jorrín-Novo |
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| Institution: | 1.Agricultural and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology,University of Córdoba,Córdoba,Spain;2.Institute of Biochemical Plant Pathology, Helmholtz Zentrum München-German Research Center for Environmental Health,Neuherberg,Germany |
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| Abstract: | Nitric oxide (NO) is a key signaling molecule in plants, being its biological effects mainly mediated through S-nitrosylation
of cysteine thiols. Using the biotin switch method combined with mass spectrometry analysis we have identified 127 targets
of S-nitrosylation in Arabidopsis cell suspension cultures and leaves challenged with virulent and avirulent isolates of Pseudomonas syringae pv. tomato. The NO targets are proteins associated with carbon, nitrogen, and sulpfur metabolism, photosynthesis, the cytoskeleton,
stress-, pathogen- and redox-related and signaling proteins. Some proteins were previously identified in plants and mammals,
while others (63%) represent novel targets of S-nitrosylation. Our data suggest that NO might be orchestrating the whole plant
physiology, presumably through covalent modification of proteins. |
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