Chaperonin containing T-complex polypeptide (CCT) subunit expression in oral mucosal wounds and fibroblasts |
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Authors: | Latha Satish Nancy Lo Phillip H. Gallo Sandra Johnson Stephanie Haberman Sandeep Kathju |
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Affiliation: | (1) Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA 15212, USA;(2) Drexel University College of Medicine, Queen Lane Medical Campus, Philadelphia, PA 19129, USA;(3) Wound Healing Program, Center for Genomic Sciences, Allegheny-Singer Research Institute, 320 East North Avenue, Pittsburgh, PA 15212-4772, USA; |
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Abstract: | Mucosal wound healing in adults has been reported to feature diminished scar formation compared to healing skin wounds. We sought to determine if the expression pattern of chaperonin containing T-complex polypeptide (CCT) subunits in mucosal wounds and fibroblasts is different from that observed in skin wounds and fibroblasts. We found that CCT-beta is the only subunit message to be reduced in wounded mucosa versus unwounded control, and this reduction was confirmed at the protein level. In contrast, mRNA levels of CCT-zeta, -delta, -eta, and -epsilon were significantly increased in mucosal wounds. The increase in CCT-eta was also confirmed at the protein level. Expression levels of CCT-alpha, -beta, -delta; -epsilon, and -theta mRNAs were significantly increased in adult mucosal fibroblasts in culture compared to skin-derived fibroblasts. Western blot analyses confirmed a modest increase in CCT-beta in adult mucosal fibroblasts relative to skin fibroblasts, but CCT-eta protein was unaffected. These differences may contribute to the reported difference in healing outcomes between these two tissue types. |
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Keywords: | Chaperonin containing T-complex polypeptide CCT Oral mucosal wounds qRT-PCR Fibroblasts |
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