An optimized procedure for the separation of amino acid phenylthiohydantoins by reversed-phase HPLC |
| |
Authors: | B Persson D Eaker |
| |
Affiliation: | Department of Biochemistry, University of Uppsala, Sweden. |
| |
Abstract: | An optimization procedure for the separation of 24 PTH-amino acids by high-performance liquid chromatography on an inexpensive Merck Superspher Si 60 RP-8, (4.0 x 250 mm) column with PTH-Nle as an internal standard is described. The effects of pH, ionic strength, temperature and gradient were investigated. Using conventional HPLC equipment, the practical detection limit is about 5 pmol. |
| |
Keywords: | |
|
|