An optimized procedure for the separation of amino acid phenylthiohydantoins by reversed-phase HPLC |
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| Authors: | B Persson D Eaker |
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| Affiliation: | Department of Biochemistry, University of Uppsala, Sweden. |
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| Abstract: | An optimization procedure for the separation of 24 PTH-amino acids by high-performance liquid chromatography on an inexpensive Merck Superspher Si 60 RP-8, (4.0 x 250 mm) column with PTH-Nle as an internal standard is described. The effects of pH, ionic strength, temperature and gradient were investigated. Using conventional HPLC equipment, the practical detection limit is about 5 pmol. |
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