N-1-napthylphthalamic-acid-binding activity of a plasma membrane-rich fraction from maize coleoptiles |
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| Authors: | Carole A. Lembi D. James Morré K. St.-Thomson R. Hertel |
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| Affiliation: | (1) Department of Botany and Plant Pathology, Purdue University, Lafayette, Indiana;(2) Department of Biological Sciences, Purdue University, Lafayette, Indiana;(3) Institut für Biologie III, Universität Freiburg i. Br., Freiburg i. Br., Germany |
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| Abstract: | Summary Plasma membrane-rich fractions were prepared from maize coleoptiles by low-shear homogenization and differential and sucrose-gradient centrifugation. Plasma membrane fragments were identified using a specific cytochemical stain based on phosphotungstic acid prepared in chromic acid. In a comparison of 10 different cell fractions of varying plasma membrane content, the N-1-napthylphthalamic-acid (NPA)-binding activity of the fractions was directly proportional to the content of plasma membrane. The NPA binding appears to be strong KM between 10-8 and 10-7 M) but non-covalent. NPA is known to inhibit auxin transport efficiently and quickly. Thus, the results are consistent with the localization of auxin transport sites at the plasma membrane of plant cells.Purdue University Agricultural Experiment Station Journal Paper No. 4355. This work was supported in part by a grant from the National Science Foundation GB-23183.Supported by National Science Foundation Postdoctoral Fellowship. |
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