Expression of the E2 open reading frame of papilloma viruses BPV1 and HPV6b in Escherichia coli |
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| Authors: | R G Mallon W Sisk V Defendi |
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| Affiliation: | 1. Department of Pathology and Kaplan Cancer Center, New York University Medical Center, 550 First Avenue, New York, NYU.S.A. 10016, Tel. (212)340-5927;1. National Cancer Institute, Frederick Cancer Research Facility, Frederick, MD 20871 U.S.A. Tel. (301) 695-1582;1. Physiology Department, School of Medicine, Tehran University of Medical Sciences, Iran;2. Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran;1. Graduate School of Science and Technology, Niigata University, 8050 Ikarashi 2-nocho, Niigata 950-2181, Japan;2. Department of Nanotechnology and Advanced Materials Engineering, Sejong University, 209 Neungdong-ro, Gwangjin-gu, Seoul 05006, Republic of Korea;3. Department of Marine Resource Science, Faculty of Agriculture and Marine Science, Kochi University, 200 Otsu, Monobe, Nankoku City, Koch 783-8502, Japan;4. Department of Chemistry and Chemical Engineering, Niigata University, 8050 Ikarashi 2-Nocho, Niigata 950-2181, Japan;5. N-Luminescence Corporation, 8867-3 Ikarashi 2-nocho, Niigata 950-2101, Japan;6. Faculty of Engineering, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8550, Japan;7. School of Advanced Materials Science and Engineering, Sungkyunkwan University, Kyunggi-do 400-746, Republic of Korea;1. Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan;2. Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan;1. Department of Entomology and Nematology, and UC Davis Comprehensive Cancer Center, University of California, Davis, CA 95616, USA;2. Department of Nutrition, The University of Tennessee, Knoxville, TN 37996, USA;3. Department of Public Health, The University of Tennessee, Knoxville, TN 37996, USA;1. Laboratório de Farmacologia e Neurobiologia, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Porto, Portugal;2. Center for Drug Discovery and Innovative Medicines (MedInUP), Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Porto, Portugal |
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| Abstract: | A new expression vector, pRA10, has been constructed for the expression of the open reading frames (ORF) of bovine (B) and human (H) papilloma viruses (PV). This vector is a derivative of pAJ pi and contains 15 restriction sites proximal to the lambda PL promoter, offering considerable versatility for insertion of different ORFs. This vector was used specifically to express the E2 ORF gene products from BPV1 and HPV6b at high level in Escherichia coli. The genuine nature of these proteins was demonstrated by restriction map analysis of expression vector plasmids to insure proper orientation, nucleotide sequence analysis to demonstrate in-frame insertion, E2 ORF protein production by expression-vector plasmids, and not by appropriate controls and, immunoprecipitation of E2 proteins by antibody specific for a common N-terminal sequence derived from the expression vector. |
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