Purification and characterization of a novel ribosome-inactivating protein from seeds of Trichosanthes kirilowii Maxim |
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Authors: | Shao-hua Shu Guo-zhen Xie Xiao-liang Guo Mo Wang |
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Institution: | aInstitute of Medicinal Plant Development, College of Plant Science and Technology, Huazhong Agricultural University, 1# Shizishan Street, Hongshan Borough, Wuhan 430070, PR China |
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Abstract: | A novel ribosome-inactivating protein, designated Trichosanthrip, was purified from mature seeds of Trichosanthes kirilowii Maxim by cation-exchange and gel-filtration chromatography. Trichosanthrip migrated as a single band in SDS–PAGE, with an apparent molecular mass of 13 kDa. The molecular mass of Trichosanthrip was 10,964.617 Da as determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Trichosanthrip showed N-glycosidase activity on 28 S rRNA and strongly inhibited cell-free protein synthesis, with an IC50 of 1.6 ng/ml. Liquid chromatography–tandem mass spectrometry showed that Trichosanthrip was a novel protein with similar sequence to other proteins present in members of the Cucurbitaceae. |
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Keywords: | Ribosome-inactivating protein N-Glycosidase activity Protein synthesis inhibitory activity Trichosanthes kirilowii Maxim |
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