Humoral stimulating activities in post-cyclophosphamide rat sera and their purified fractions |
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| Authors: | N Pujic Lj Hegedis K Pavelic T Casl S Marusic K Savovski A Dujic B Dimitrijevic |
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| Institution: | Laboratory for Radiology and Radiation Medicine, Institute of Nuclear Sciences, Vinca, Belgrade;Rudjer Boskovic Institute, Zagreb, Faculty of Pharmacology, University of Zagreb, Zagreb;†Military-Medical Academy, Belgrade, Yugoslavia |
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| Abstract: | Abstract. Enhanced humoral, stimulating activities (HSAs) of post-cyclophosphamide (CY) sera and their purified fractions, acting on mitogen activated T-lymphocytes, were detected in Wistar rats after treatment by high single doses of the aplasia producing alkylating cytostatic drug CY. These activities, monitored in vitro , were partially purified from post-CY sera, collected 2, 4, and 7 days after treatment, yielding fractions with higher humoral stimulating activity. The preliminary purification included molecular cutting by Amicon-Diaflo filters (1-30 kDa pore size range) and gel-filtration on Sephadex G-50 and G-75. Results show that post-CY sera partially purified fraction (10–20 kDa), enhances the proliferation of hydrocortisone resistant (HCR) T-lymphocytes up to threefold under microculture conditions ( P «0.001); partially purified post-CY sera fraction (10–20 kDa) increases the proliferative response of T-cells in microcultures to the mitogenic 145–2C11 monoclonal antibody (mAb) up to tenfold ( P «0.001). These results show that the activity of stimulating factors is localized in the molecular weight range of 10–20 kDa. |
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