| 从白腐菌中筛选漆酶高产菌株的初步研究 |
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| 引用本文： | 唐舜 A.LOMASCOLO 郭林 J.C.SIGOILLOT 文华安 卯晓岚 王文惠 J.L.ROBERT C.ANTONA M.ASTHER. 从白腐菌中筛选漆酶高产菌株的初步研究[J]. 菌物学报, 2001, 20(4): 520-525 |
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| 作者姓名： | 唐舜 A.LOMASCOLO 郭林 J.C.SIGOILLOT 文华安 卯晓岚 王文惠 J.L.ROBERT C.ANTONA M.ASTHER |
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| 作者单位： | 1. 中国科学院微生物研究所
2. Unité INRA de Biotechnologie des Champignons Filamenteux, IFR de Biotechnologie Agro-lndustrielle de Marseille, ESIL, Universités de Provence et de la Méditerranée, |
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| 基金项目： | Supported by the EuropeanCommission DG Ⅻ-INCO DC,No.ERBIC18CT980285 and ChineseFrench cooperation programE97-01.(欧盟和中法先进研究计划项目) |
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| 摘 要： | 从中国分离的149株木腐菌中,有30株具有漆酶活性。在添加了阿魏酸的液体培养基中,漆酶产量高(每升培养液中漆酶含量在10000单位以上)的菌株有9个:游离细胞培养的彩绒革盖菌Coriolus versicolor G30,相邻小孔菌Microporus affinis G07,血红密孔菌Pycnoporus sanguineus W006,W006-2,W3008,G05和香菇Lentinus edodes G18,以及固定细胞培养的鲑贝革盖菌Coriolus consors 98563和干酪菌Tyromyces sp. 98420。彩绒革盖菌、相邻小孔菌和血红密孔菌具有重要的生物工程研究价值。
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| 关 键 词： | 木腐菌 漆酶活性 游离细胞培养 固定细胞培养 密孔菌Pycnoporus 革盖菌Coriolus 小孔菌Microporus |
| 文章编号： | 1007-3515(2001)04-0520-06 |
| 修稿时间： | 2001-02-07 |
ISOLATION OF WHITE-ROT FUNGI FROM CHINA FOR SCREENING LACCASE-HYPERPRODUCINGSTRAINS |
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| TANG Shun LOMASCOLO Anne GUO Lin SIGOILLOT Jean-Claude WEN Hua-An MAO Xiao-Lan WANG Wen-Hui ROBERT Jean-Luc ANTONA Claudine ASTHER Marcel. ISOLATION OF WHITE-ROT FUNGI FROM CHINA FOR SCREENING LACCASE-HYPERPRODUCINGSTRAINS[J]. Mycosystema, 2001, 20(4): 520-525 |
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| Authors: | TANG Shun LOMASCOLO Anne GUO Lin SIGOILLOT Jean-Claude WEN Hua-An MAO Xiao-Lan WANG Wen-Hui ROBERT Jean-Luc ANTONA Claudine ASTHER Marcel |
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| Affiliation: | TANG Shun1 LOMASCOLO Anne2 GUO Lin1 SIGOILLOT Jean-Claude2 WEN Hua-An1 MAO Xiao-Lan1 WANG Wen-Hui1 ROBERT Jean-Luc2 ANTONA Claudine2 ASTHER Marcel |
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| Abstract: | The isolation of wood-decay fungi from China for screening laccase-producing strains was carried out. One hundred and forty-nine strains were isolated and 30 strains with a basal laccase activity were studied for laccase hyperproduction in liquid medium supplemented with ferulic acid. The highest laccase activities (over 10000 U/l) were obtained in free-cell cultures of Coriolus versicolor G30, Microporus affinis G07, Pycnoporus sanguineus W006, P. sanguineus W006-2, P. sanguineus W3008, P. sanguineus G05 and Lentinus edodes G18 and in immobilized-cell cultures of Tyromyces sp. 98420 and Coriolus consors 98563. The species C. versicolor, M. affinis and P. sanguineus were of particular biotechnological interest. |
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| Keywords: | Wood-decay fungi laccase activity free-cell culture immobilized-cell culture Pycnoporus Coriolus Microporus |
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