Multiple mechanisms contribute to myenteric plexus ablation induced by benzalkonium chloride in the guinea-pig ileum |
| |
Authors: | Edward J Parr Keith A Sharkey |
| |
Institution: | (1) Neuroscience Research Group, Department of Physiology and Biophysics, The University of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta, Canada T2N 4N1, CA |
| |
Abstract: | Ablation of rat myenteric plexus with benzalkonium chloride has provided a model of intestinal aganglionosis, but the degenerative
responses are not well understood. We examined the effects of this detergent on neurons and glia, including expression of
c-Myc, c-Jun, JunB, and c-Fos, and on immunocytes in the guinea-pig ileum. Benzalkonium chloride (0.1%) or saline was applied
to the serosal surface of distal ileum. Tissues were analyzed 2, 3, or 7 days later and compared with cyclosporine-treated
and untreated animals. More than 90% of myenteric neurons were destroyed in ileal segments 3–7 days after benzalkonium-chloride
treatment. Glia withdrew processes from around neurons after 2 days and were mostly gone after 3 days. Neuronal c-Myc began
to disappear while c-Fos, c-Jun, and JunB were evident in some neuronal nuclei after 2 or 3 days. After 3 days, widespread
apoptosis was evident in the myenteric plexus. Populations of T cells, B cells, and macrophage-like cells in untreated and
saline-treated myenteric plexuses were substantially increased 3 and 7 days after benzalkonium-chloride treatment. Cyclosporine
delayed significant neuronal loss. We conclude that a variety of degenerative mechanisms may be active in this model, including
an immune response which may actively contribute to tissue destruction.
Received: 13 September 1996 / Accepted: 20 January 1997 |
| |
Keywords: | : Myenteric plexus Benzalkonium chloride Immunohistochemistry Lymphocytes Macrophages Glia Oncoproteins Guinea pig (Rodentia) |
本文献已被 SpringerLink 等数据库收录! |
|