Synthesis and assembly of a functionally active recombinant platelet-derived growth factor AB heterodimer |
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| Authors: | A Ostman L Rall A Hammacher M A Wormstead D Coit P Valenzuela C Betsholtz B Westermark C H Heldin |
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| Affiliation: | Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden. |
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| Abstract: | A Chinese hamster ovary cell line that stably expresses transfected human platelet-derived growth factor (PDGF) A and B chain precursors was established. All three dimeric combinations of PDGF chains were produced by this cell line; their biosynthesis, assembly, and processing were followed by pulse-chase analyses. PDGF-AA, PDGF-AB, and PDGF-BB were processed to Mr values of about 30,000 and were accumulated in these forms in the medium. In addition, PDGF-BB was further processed to a 24-kDa component, which remained cell-associated. The major secreted component was PDGF-AB, which was purified and shown to have structural and functional characteristics indistinguishable from PDGF-AB purified from human platelets. |
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