Fermentation process for continuous production of succinic acid in a fibrous bed bioreactor |
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| Institution: | 1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, People''s Republic of China;2. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, People''s Republic of China;1. Metabolic Engineering Research Unit, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand;2. Division of Biopharmacy, Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand;1. DSM Biotechnology Center, Alexander Fleminglaan 1, 2613 AX Delft, The Netherlands;2. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands;1. School of Energy and Environment, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China;2. Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, Guangdong 518120, China;3. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, No. 2, Nengruan Road, Wushan, Tianhe, Guangzhou, Guangdong, 510640, China;4. School of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou, 450001, China;1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, Jiangsu 211800, China;2. Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing, Jiangsu 211800, China;3. Renewable and Sustainable Energy Institute (RASEI), University of Colorado Boulder, 4001 Discovery Dr. Suite N321, Boulder 80309, USA;1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;2. University of the Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, China;3. China National Center for Biotechnology Development, Beijing 100036, China |
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| Abstract: | Succinic acid (SA) was produced from Actinobacillus succinogenes with high cell density by continuous fermentation using fibrous bed bioreactor (FBB). The effects of feeding glucose concentration, dilution rate, and pH on continuous production of SA were examined to achieve an efficient and economical bioprocess. The optimum feeding glucose concentration, dilution rate, and pH were 80 g/L, 0.05 1/h, and 6.0–6.5, respectively. A SA concentration of 55.3 ± 0.8 g/L, productivity of 2.77 ± 0.04 g/L/h, and yield of 0.8 ± 0.02 g/g were obtained, and the continuous fermentation exhibited long-term stability for as long as 18 days (440 h) with no obvious fluctuations in both SA and biomass levels. The Jerusalimsky equation for the specific rate of SA production presented the inhibition phenomenon of the product, demonstrating that 60 g/L SA might be a critical concentration in this continuous FBB system. The results obtained could be beneficial for future fermentor designs and improvements in SA production. |
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| Keywords: | Succinic acid Bioprocess design Bioreactors Fermentation Immobilized cells |
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