Stability of oxidative stress biomarkers in flathead mullet,Mugil cephalus,serum during short-term storage |
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| Institution: | 1. Department of Veterinary Sciences, University of Messina, Polo Universitario dell’Annunziata, 98168 Messina, Italy;2. Department of Sciences, University of Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, Italy;3. Department of Biological and Environmental Sciences, University of Messina, Viale Ferdinando Stagno d''Alcontres 31, 98166, S. Agata, Messina, Italy;4. Consiglio per la Ricerca e la Sperimentazione in Agricoltura – Unità di Ricerca per la Zootecnia Estensiva, Via Appia, 85054 Muro Lucano, Potenza, Italy;1. Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Columbus Center, 701 E. Pratt Street, Baltimore, MD 21202, USA;2. College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian 361102, China;1. Department of Neurology, School of Medicine, Yildirim Beyazit University, Ankara, Turkey;2. Department of Neurology, Atatürk Training and Research Hospital, Ankara, Turkey;3. Department of Clinical Biochemistry, Atatürk Training and Research Hospital, Ankara, Turkey;4. Department of Clinical Biochemistry, School of Medicine, Yildirim Beyazit University, Ankara, Turkey;1. “Torre Flavia” LTER (Long Term Ecological Research) Station, Protected area Service, Città Metropolitana di Roma Capitale, via Tiburtina, 691, 00159 Rome, Italy;2. Laboratorio di Aerobiologia, Dipartimento di Biologia, Seconda Università di Roma Tor Vergata, via della Ricerca Scientifica, 00133 Rome, Italy;3. Regione Lazio, Direzione Ambiente e sistemi naturali, via del Pescaccio, 96-00166 Rome, Italy;1. Agrolab Danmark A/S, Rojleskovej 18, DK-5500 Middelfart, Denmark;2. Institut Méditerranéen de Biodiversité et d’Ecologie marine et continentale, IMBE UAPV AMU IRD, Pôle Agrosciences, 301 rue Baruch de Spinoza, BP 21239, 84916 Avignon, France;3. Université Catholique de l’Ouest, 3 Place André Leroy, 49100 Angers, France;4. INRA Avignon, Site Agroparc, 84914 Avignon Cedex 09, France |
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| Abstract: | The production of reactive oxygen species (ROS) by aquatic organisms in response to stressful factor is a common feature in aquatic environment. Thus, the evaluation of parameters related to oxidative stress in fish, in first instance those showing the redox potential, is notable to characterize the environmental conditions.Short-term storage of samples is essential when blood must be transported from collection sites to laboratories. Therefore, excessive delays in processing might compromise the reliability of results. The aim of the present study was to assess the effect of short-term storage time at +4 °C on total oxidant status (TOS), total antioxidant capacity (TAC) and oxidative stress index (OSI) in flathead mullet (Mugil cephalus) serum. After blood collection, all sample were analyzed and the assays were repeated after 24, 48 and 72 h from sampling. results showed a significant effect of short-term storage on TOS and TAC (P ≤ 0.0001), while the statistical significance of the linear regression study for these parameters was reduced as consequence of storage. These results highlight that the activities of oxidants and antioxidants in flathead mullet serum change during short-term storage at 4 °C and should be assesses as soon as possible from collection. |
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| Keywords: | Fish Oxidative Stress Index Total antioxidant status Total oxidant status |
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