Quantitative modeling of inducer transport in fed-batch cultures of Escherichia coli |
| |
| Affiliation: | 1. Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China;2. Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts AL9 7TA, UK;1. School of Biosciences, SRM University, Chennai, Tamil Nadu, India;2. Department of Biotechnology, Sathyabama University, Chennai, Tamil Nadu, India;3. SynkroMax Biotech Pvt. Ltd., Chennai, Tamil Nadu, India |
| |
| Abstract: | An unsteady, unstructured, unsegregated and based on first principles mathematical model has been proposed to describe IPTG (isopropyl-β-d-tiogalactopiranoside) transport in induced fed-batch cultures of E. coli M15 ΔglyA [pQEαβrham] [pREP4] producing rhamnulose 1-phosphate aldolase (RhuA). The model predicts extracellular and intracellular IPTG concentration. Experimental extracellular IPTG concentrations under different operational conditions were obtained by HPLC–MS analysis. These experimental data were used to fit the parameters of the model. The model was also able to predict the experimental behavior of two different E. coli strains producing fuculose 1-phosphate aldolase (FucA). IPTG transport to cells was the contribution of three processes: a diffusion process, and two active processes (one non-specific and another specific). |
| |
| Keywords: | Mathematical model Inducer transport IPTG Lac permeases |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
