Genetic structure of Pseudo-nitzschia pungens (Bacillariophyceae) populations: Implications of a global diversification of the diatom |
| |
| Affiliation: | 1. Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia;2. Bachok Marine Research Station, Institute of Ocean and Earth Sciences, University of Malaya, 16310 Bachok, Kelantan, Malaysia;3. Fisheries and Oceans Canada, Gulf Fisheries Centre, PO Box 5030, Moncton, NB, Canada E1C 9B6;1. Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, Kota Samarahan, 94300 Sarawak, Malaysia;2. Bachok Marine Research Station, Institute of Ocean and Earth Sciences, University of Malaya, Bachok, 16310 Kelantan, Malaysia;3. Institute of Oceanography, VAST, 01 Cau Da, Nha Trang, Vietnam;4. Fisheries and Oceans Canada, Gulf Fisheries Centre, P.O. Box 5030, Moncton, NB, Canada E1C 9B6;1. Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory of Healthy and Safe Aquaculture, College of Life Science, South China Normal University, West 55 of Zhongshan Avenue, Guangzhou, 510631, PR China;2. Natural History Museum of Denmark, University of Copenhagen, Øster Farimagsgade 5, 1307, Copenhagen K, Denmark;3. Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, Kota Samarahan, 94300, Sarawak, Malaysia;4. Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, PR China;5. Bachok Marine Research Station, Institute of Ocean and Earth Sciences, University of Malaya, 16310, Bachok, Kelantan, Malaysia;1. Normandie Univ, UNICAEN, CNRS, BOREA, 14000 Caen, France;2. UMR BOREA, CNRS-7208, IRD-207, MNHN, UPMC, UCBN, 14032 Caen, France;3. CMAbio-SF 4206 ICORE UNICAEN, 14032 Caen, France;4. Ifremer, LER/N, 14520 Port en Bessin, France;5. LABEO Frank Duncombe, Saint-Contest, 14053 Caen Cedex, France |
| |
| Abstract: | Pseudo-nitzschia pungens is a planktonic marine diatom known to be widespread in tropical and temperate coastal waters. We examined the population genetic structure of tropical Southeast Asian populations of P. pungens and compared it with those of northern and southern temperate populations. The secondary structures of the nuclear encoded internal transcribed spacer (ITS) region of 164 strains of P. pungens were modeled and analyzed. The tree revealed three ITS entities: clade I (comprised of P. pungens var. pungens) was distributed mainly in northern temperate waters; clade II (comprised of both P. pungens var. pungens and var. cingulata) was mainly from the NE Pacific; and clade III (comprised of both P. pungens var. pungens and var. aveirensis) was restricted to tropical and warm-temperate waters. Hybrids of both P. pungens var. pungens and var. cingulata co-occurred in clades I and II. Sixty haplotypes were revealed from the sequences of 164 strains. Haplotype diversity inferred from the median-joining network was in accordance with phylogenetic analysis, further supporting the grouping of the P. pungens haplogroups. Our results revealed limited gene flow between P. pungens from tropical and temperate waters, and significant population structure, as estimated by an analysis of molecular variance (AMOVA), with 75% of the total ITS variation found among populations (ФST = 0.75). This study suggests that distinct environmental clines, such as ocean thermohaline circulation, have a potential for fragmenting and dispersing global populations of P. pungens. Formation of the Isthmus of Panama, in particular, is speculated to play a role in this allopatric differentiation in P. pungens populations worldwide. |
| |
| Keywords: | Diversification ITS secondary structure Phylogeography Population structure |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
