Immobilization of porcine pancreatic lipase on poly-hydroxybutyrate particles for the production of ethyl esters from macaw palm oils and pineapple flavor |
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| Institution: | 1. Laboratory of Biocatalysis, Federal University of São João del-Rei, 35701-970 Sete Lagoas, MG, Brazil;2. Department of Chemical Engineering, Engineering School of Lorena, University of São Paulo, 12602-810 Lorena, SP, Brazil;3. Institute of Chemistry, Federal University of Alfenas, 37130-000 Alfenas, MG, Brazil;1. School of Chemical Engineering and Technology, Hebei University of Technology, 8 Guangrong Road, Hongqiao District, Tianjin, 300130, PR China;2. Tianjin Sino-German University of Applied Sciences, Tianjin, 300350, PR China;1. Faculty of Chemical Engineering, Noshirvani University of Technology, Babol, Iran;2. Department of Pharmacology and Physiology, School of Medicine, Babol University of Medical Sciences, Babol, Iran;3. School of Chemical Engineering, Engineering Campus, UniversitiSains Malaysia, Seri Ampangan, 14300 NibongTebal, SeberangPrai Selatan, Pulau Pinang, Malaysia;1. Biotechnology, Bioprocess and Biocatalysis Group, Institute of Food Science and Technology, Federal University of Rio Grande do Sul State, Av. Bento Gonçalves, 9500, PO Box 15090, ZC 91501-970 Porto Alegre, RS, Brazil;2. Department of Biocatalysis, ICP-CSIC, Campus UAM-CSIC, Cantoblanco, ZC 28049 Madrid, Spain;1. Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, CEP, 60455-760 Fortaleza, CE, Brazil;2. Departamento de Química Orgânica e Inorgânica, Laboratório de Biotecnologia e Síntese Orgânica (LABS), Universidade Federal do Ceará, Campus do Pici, Caixa Postal 6044, 60455-970 Fortaleza, CE, Brazil;3. ICP-CSIC, Campus UAM-CSIC, Cantoblanco, 28049 Madrid, Spain;4. Instituto de Engenharias e Desenvolvimento Sustentável, Universidade da Integração Internacional da Lusofonia Afro-Brasileira, CEP 62785-000, Acarape, CE, Brazil;1. Laboratory of Natural Substances, National Institute of Research and Physical and Chemical Analysis (INRAP), Biotechnopôle Sidi Thabet, 2020 Ariana, Tunisia;2. Biocatalysis and Industrial Enzymes Group, Laboratory of Microbial Ecology and Technology, National Institute of Applied Sciences and Technology (INSAT) Tunis, University of Carthage, Tunisia |
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| Abstract: | Poly-hydroxybutyrate particles (PHB) were used as support to immobilize porcine pancreatic lipase (PPL). The biocatalysts prepared were tested in the synthesis of pineapple flavor by esterification of butanol and butyric acid in heptane medium, and in the synthesis of ethyl esters by transesterification of macaw palm pulp (MPPO) and macaw palm kernel (MPKO) oils with ethanol in solvent-free systems. The effect of protein loading on the biocatalyst activity was assessed in olive oil hydrolysis. Maximum hydrolytic activity of 292.8 ± 8.60 IU/g was observed. Langmuir isotherm model was applicable to the adsorption of PPL on PHB particles. Maximum immobilized protein amount was 24.3 ± 1.70 mg/g. The optimal pH and temperature in hydrolysis reaction for the immobilized PPL were at pH 8.5 and 50 °C, while for the crude PPL extract were at pH 8.0 and 45 °C. Immobilized PPL exhibited full hydrolytic activity after 2 h of incubation in non-polar solvents. In esterification reaction, optimal conversion was around 93% after 2 h of reaction. After six esterification cycles, the biocatalyst retained 63% of its initial activity. The biocatalyst prepared attained transesterification yield of 50% after 48 h of reaction for MPKO and 35% after 96 h of reaction for MPPO. |
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| Keywords: | Porcine pancreatic lipase Immobilization Physical adsorption Poly-hydroxybutyrate particle Pineapple flavor Ethyl ester |
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