Metabolism of DNA secondary structures at the eukaryotic replication fork |
| |
Institution: | 1. Biochemistry Ph.D. Program, Florida International University, 11200 SW 8th Street, Miami, FL 33199, United States;2. Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th Street, Miami, FL 33199, United States;3. Biomolecular Sciences Institute, School of Integrated Sciences and Humanities, Florida International University, 11200 SW 8th Street, Miami, FL 33199, United States;1. Univ. Bordeaux, ARNA Laboratory, F-33000 Bordeaux, France;2. INSERM U869, IECB, F-33600 Pessac, France;1. Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA;1. Department of Microbiology, Health Sciences Building – J-wing, University of Washington, Seattle, WA, 98195;2. Molecular and Cellular Biology Program, University of Washington, Seattle, WA, 98195;3. Department of Genome Sciences, Foege Building, University of Washington, Seattle, WA 98195 |
| |
Abstract: | DNA secondary structures are largely advantageous for numerous cellular processes but can pose specific threats to the progression of the replication machinery and therefore genome duplication and cell division. A number of specialized enzymes dismantle these structures to allow replication fork progression to proceed faithfully. In this review, we discuss the in vitro and in vivo data that has lead to the identification of these enzymes in eukaryotes, and the evidence that suggests that they act specifically at replication forks to resolve secondary structures. We focus on the role of helicases, which catalyze the dissociation of nucleotide complexes, and on the role of nucleases, which cleave secondary structures to allow replication fork progression at the expense of local rearrangements. Finally, we discuss outstanding questions in terms of dismantling DNA secondary structures, as well as the interplay between diverse enzymes that act upon specific types of structures. |
| |
Keywords: | DNA secondary structures Replication fork Hairpins Trinucleotide repeats G-quadruplexes Telomere loops Fork reversal Helicase Nuclease |
本文献已被 ScienceDirect 等数据库收录! |
|