Recycling biodiesel-derived glycerol by the oleaginous yeast Rhodosporidium toruloides Y4 through the two-stage lipid production process |
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| Institution: | 1. Dalian University of Technology, Dalian 116024, PR China;2. Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, CAS, Dalian 116023, PR China;3. University of Chinese Academy of Sciences, Beijing 100049, PR China;1. Department of Biology, Massachusetts Institute of Technology, Cambridge, 02139 MA, USA;2. Biomaterials Science & Engineering Laboratory, Massachusetts Institute of Technology, Cambridge, 02139 MA, USA;3. Engineering Systems Division, Massachusetts Institute of Technology, Cambridge, 02139 MA, USA;1. International Center for Biotechnology, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan;2. Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand;1. Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand;2. Center for Advanced Studied in Tropical Natural Resources, National Research University-Kasetsart University, Chatuchak, Bangkok 10900, Thailand;3. International Center for Biotechnology, Osaka University, Suita-shi, Osaka 565-0871, Japan;1. Micalis Institute, Institut National de la Recherche Agronomique (INRA), AgroParisTech, Université Paris–Saclay, 78350 Jouy-en-Josas, France;2. Department of Bioengineering, Imperial College London, London SW7 2AZ, UK;1. Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 2N2, Canada;2. Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada;1. School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou, 510006, China;2. School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, 510006, China;3. Analysis and Test Center, Guangdong University of Technology, Guangzhou, 510006, China |
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| Abstract: | Direct utilization of crude glycerol, a major byproduct in biodiesel industry, becomes imperative, because its production has outpaced the demand recently. We demonstrated that the oleaginous yeast Rhodosporidium toruloides Y4 had a great capacity to convert glycerol into lipids with high yield using the two-stage production process. Significantly higher cell mass and lipid yield were observed when the media were made with synthetic crude glycerol than pure glycerol. The process achieved a lipid yield of 0.22 g g−1 glycerol, which was comparable with the lipid yield using glucose as the substrate. Lipid samples showed similar fatty acid compositional profiles to those of vegetable oils, suggesting that such microbial lipids were potential feedstock for biodiesel production. Our data provided an attractive route to integrate biodiesel production with microbial lipid technology for better resource efficiency and economical viability. |
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| Keywords: | Bioconversion Biodiesel Glycerol Microbial growth Optimization Oleaginous yeast |
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