Production of butyric acid by a cellulolytic actinobacterium Thermobifida fusca on cellulose |
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| Institution: | 1. State Key Laboratory of Marine Geology, Tongji University, Shanghai 200092, China;2. Department of Marine Sciences, University of Georgia, Athens, GA 30602, United States;3. State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Beijing 100083, China;4. Department of Earth and Planetary Sciences, Northwestern University, Evanston, IL 60208, United States;5. Department of Geology and Environmental Earth Science, Miami University, Oxford, OH 45056, United States;6. Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China;7. Department of Biological Sciences, University of North Carolina, Charlotte, NC 28223, United States;8. School of Life Science, University of Nevada Las Vegas, Las Vegas, NV 89154, United States |
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| Abstract: | Thermobi?da fusca not only produces cellulases, hemicellulases and xylanases, but also excretes butyric acid. In order to achieve a high yield of butyric acid, the effect of different carbon sources: mannose, xylose, lactose, cellobiose, glucose, sucrose and acetates, on butyric acid production was studied. The highest yield of butyric acid was 0.67 g/g C (g-butyric acid/g-carbon input) on cellobiose. The best stir speed and aeration rate for butyric acid production were found to be 400 rpm and 2 vvm in a 5-L fermentor. The maximum titer of 2.1 g/L butyric acid was achieved on 9.66 g/L cellulose. In order to test the production of butyric acid on lignocellulosic biomass, corn stover was used as the substrate, on which there was 2.37 g/L butyric acid produced under the optimized conditions. In addition, butyric acid synthesis pathway was identified involving five genes that catalyzed reactions from acetyl-CoA to butanoyl-CoA in T. fusca. |
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| Keywords: | Fermentation Batch processing Cellulase Cellulose Butyric acid |
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