Anaerobic toluene activation by benzylsuccinate synthase in a highly enriched methanogenic culture |
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Authors: | Beller H R Edwards E A |
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Institution: | Lawrence Livermore National Laboratory, Livermore, California 94551-0808, USA. beller2@llnl.gov |
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Abstract: | Permeabilized cells of a highly enriched, toluene-mineralizing, methanogenic culture catalyzed the addition of toluene to fumarate to form benzylsuccinate under anaerobic conditions. The specific in vitro rate of benzylsuccinate formation was >85% of the specific in vivo rate of toluene consumption. This is the first report of benzylsuccinate synthase activity in a methanogenic culture; the activity has previously been reported to occur in denitrifying, sulfate-reducing, and anoxygenic phototrophic bacteria. |
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