The role of auxins in differentiation of rice tissues culturein Vitro |
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Authors: | Toyoyuki Nishi Yasuyuki Yamada Eiichi Takahashi |
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Institution: | (1) Department of Agricultural Chemistry, Kyoto University, 606 Kyoto;(2) Present address: The Nippon, Shinyaku Institute for Botanical Research, Oyake, Yamashina, 607 Kyoto |
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Abstract: | Effects of various auxins on callus induction (dedifferentiation) and organ redifferentiation from the callus were studied
by using various tissues of rice,Oryza sativa L. cv. Kyoto Asahi.
2,4-D, NAA and IAA were used as auxins for the test of their ability to induce callus. All of these were active. This callus
induction by auxin was successful in all tissues used; seed, root, shoot nodule, anther and ovary.
In all of the calluses induced by various auxins such as 2,4-D, NAA and IAA and derived from various tissues such as seed,
root, shoot nodule, anther and ovary, organ redifferentiation, i.e., formation of shoots and roots was achieved by removing
the auxins from the medium used for the callus calture. Cytokinins were not necessary for the organ redifferentiation in these
calluses.
These results suggest that auxin is the only exogenous factor that determines dedifferentiation and redifferentiation in rice
plant tissues culturedin vitro. |
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Keywords: | |
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