The human lymphocyte micronucleus assay: a comparison between whole-blood and separated-lymphocyte cultures |
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| Authors: | L Migliore M Nieri S Amodio N Loprieno |
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| Affiliation: | Dipartimento di Scienze dell'Ambiente e del Territorio, Università di Pisa, Italy. |
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| Abstract: | The induction of micronuclei (MN) by vincristine, mitomycin C and cyclophosphamide was compared in purified lymphocytes and in whole-blood cultures. With both assays, cytokinesis was blocked by cytochalasin B and MN were only scored in binucleate cells. The data suggest that whole-blood cultures may be considered a better experimental condition for the detection of MN induced by chemicals in vitro. |
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