Biosynthesis of methionine in an ethionine-resistant strain ofCandida utilis |
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Authors: | Marie Musílková Z. Fencl |
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Affiliation: | 1. Department of Technical Microbiology, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 4
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Abstract: | The strainCandida utilis T 20 adapted to a high concentration of ethionine, excretes considerable amounts of methionine in a synthetic medium, about 40 times as much as the original non-adapted strain. At the same time, the amount of methionine in yeast cells incrncreased, predominantly in the pool (9 times as much as in the control). This ability to produce greater amounts of methionine in the pool or to excrete it into the medium is not permanent, since after 5 passages on agar with ut ethionine the amount of methionine was practically not increased as compared with the original non-adapted strain. An increase in free methionine and of methionine excreted into the medium was found on cultivating the strain in a molasses-containing medium, too. |
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